中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (2): 215-220.doi: 10.3969/j.issn.2095-4344.2017.02.010

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

卡拉胶/聚乙烯醇复合材料的制备及生物相容性评价

崔  菁1，张亚彬2，马思琪1，熊艳杰3，崔  曼1，李硕峰1，车鹏程1，姚芳莲2，孙  红1
  

  1. 1华北理工大学基础医学院，河北省唐山市  063000；2天津大学化工学院高分子科学与工程系，天津市  300072；3华北理工大学附属医院，河北省唐山市  063000
  • 收稿日期:2016-10-27 出版日期:2017-01-18 发布日期:2017-02-27
  • 通讯作者: 孙红，华北理工大学基础医学院，河北省唐山市 063000
  • 作者简介:崔菁，女，1990年生，河北省深州市人，汉族，华北理工大学基础医学院在读硕士，主要从事骨、软骨组织工程方面的研究。
  • 基金资助:

    国家自然科学基金(81101448/51573127)；河北省自然科学基金(C2011401006/H2012401017)；河北省引进留学人员资助项目(C201400560)

Preparation of polyvinyl alcohol/lota-carrageenan scaffolds and its biocompatibility

Cui Jing1, Zhang Ya-bin2, Ma Si-qi1, Xiong Yan-jie3, Cui Man1, Li Shuo-feng1, Che Peng-cheng1, Yao Fang-lian2,Sun Hong1
  

  1. 1Basic Medical School, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 3the Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei Province China
  • Received:2016-10-27 Online:2017-01-18 Published:2017-02-27
  • Contact: Sun Hong, Basic Medical School, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • About author:Cui Jing, Studying for master’s degree, Basic Medical School, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81101448, 51573127; the Natural Science Foundation of Hebei Province, No. C2011401006, H2012401017; the Project of Overseas Students Introduction in Hebei Province, No. C201400560

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被引次数

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文题释义：
聚乙烯醇水凝胶：具有化学性能稳定、有黏弹性，生物相容性好及加工过程简单，在生物医药领域有着广泛的应用，比如伤口敷料，口服药物和软骨组织工程。尤其是其易成型及与自然关节软骨相似的结构和生物摩擦学特性，避免过早退变和降解，被认为是关节软骨良好的替代材料。但聚乙烯醇水凝胶也存在着无生物活性的缺点，聚乙烯醇水凝胶用于组织工程存在抗蛋白的问题，这导致细胞黏附率低，并且经过冻干处理所得到的支架存在形状皱缩的现象，严重制约了其应用范围。
卡拉胶：是一种红藻提取物，作为亲水的天然多糖，由于主链中的硫酸二糖结构十分类似于天然细胞外基质成分黏多糖，因此在组织工程领域十分具有潜力。卡拉胶制备的凝胶可以很好的促进封装细胞的生长和分化。有研究结果表明支架材料中加入卡拉胶成分有益于促进三维细胞培养，从而应用在软骨组织修复领域中。

背景：聚乙烯醇水凝胶具有与天然关节软骨类似多孔结构和力学性能，适合于关节软骨修复的研究。但纯聚乙烯醇水凝胶不仅缺乏细胞黏附性，而且成型过程中容易出现形貌皱缩的现象，严重限制了其在组织工程领域中的应用。将活性聚合物添加到聚乙烯醇水凝胶体系中能够提高材料的细胞黏附能力。
目的：制备含不同卡拉胶配比的聚乙烯醇/ι-卡拉胶复合材料，以血管内皮细胞评价其生物相容性。
方法：制备含不同卡拉胶的聚乙烯醇/ι-卡拉胶二维复合膜材料，将血管内皮细胞与复合膜材料共培养，通过细胞黏附及增殖实验，扫描电镜观察细胞在材料上的生长及增殖状况，评价其生物相容性；在此基础上进一步制备不同卡拉胶配比的三维支架材料，根据医疗器械生物学评价标准进行溶血实验，扫描电镜观察复合支架材料的孔隙率、孔径。
结果与结论：①体外细胞实验结果表明卡拉胶的加入可以显著提高聚乙烯醇水凝胶的生物活性，促进细胞在材料上的黏附和增殖；②不同卡拉胶配比的各实验组溶血率均小于公认的5%，说明材料符合医用材料的溶血实验要求；③卡拉胶质量分数为20%-30%的材料有适合细胞生长、增殖的孔径和有助于养料和代谢产物的输送孔隙率，可为进一步制备组织工程支架材料提供理论支持。

关键词: 生物材料, 软骨生物材料, 复合材料, 关节软骨, 聚乙烯醇, 卡拉胶, 血管内皮细胞, 生物相容性, 国家自然科学基金

Abstract:

BACKGROUND: Polyvinyl alcohol (PVA) hydrogel with similar porous structure and mechanical properties to the natural cartilage is very suitable for the repair of articular cartilage. However, the pure PVA hydrogel after lyophilization will be accompanied by the shrinkage of the polymer network and the collapse of the pores, leading to the inhomogeneous performance of the material even in the state of re-swelling. Addition of the active polymer will increase the cell adhesion ability of PVA hydrogel.
OBJECTIVE: To construct PVA/ lota-carrageenan (l-CA) composite materials with different mass fractions of l-CA and evaluate the biocompatibility with vascular endothelial cells.
METHODS: PVA/l-CA composite films with different contents of l-CA were fabricated and then co-cultured with vascular endothelial cells. Attachment, proliferation and morphological changes of vascular endothelial cells on the composite were observed by scanning electron microscope and MTT assay to evaluate its biocompatibility. PVA/l-CA three-dimensional scaffold with different contents of l-CA were constructed, and hemolysis experiment was conducted according to the biological evaluation standards of medical devices, and the porosity and pore size were observed using scanning electron microscope.
RESULTS AND CONCLUSION: In vitro experimental results showed that the addition of l-CA could significantly increase the biological activity of PVA hydrogel, and promote the cell attachment and proliferation on the scaffold. The hemolysis rate of each experimental group was less than 5% (the accepted safety standard), suggesting that the composite materials were in accordance with the standard of medical devices for hemolysis experiment. These findings indicate that the composite scaffolds with 20%-30% l-CA possess the pore size suitable for cell growth and proliferation and the porosity beneficial for transportation of nutrients and metabolites, which can serve as an excellent scaffold for tissue engineering.

Key words: Endothelial Cells, Hydrogel, Biocompatible Materials, Tissue Engineering

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